Biotechnology

Question

Is it like first the DNA from bacteria is removed then it is ligated with gene of interest, again the DNA is introduced in the same bacteria? And if not then how did the host accepts r-DNA if the r-DNA does not have a single portion of the host DNA?

Answer 1

YEP! YOU ARE RIGHT. ..

But the plasmid/bacteria. .which is selected as a vector. ..must have high copy number origin of replication restriction sites such tetracycline, ampicillin, etc.

SO THAT THEY CAN MULTIPLY IN THE HOST CELL EASILY AND PRODUCE HIGH COPY NUMBER. 

FOR EXAMPLE:-

A bacteria named agrobacterium tumificians cause crown ball disease in plant ...these bacteria were isolated and desired genes were introduced in it..in place of disease-causing gene...

HOPE IT WILL HELP YOU..

(Source: Ans by JuhiJha via Brainly)